miR-M7-5p靶向调控马立克病病毒原癌基因meq的表达

doi:10.11843/j.issn.0366-6964.2014.09.019

Abstract

Abstract:

In the viral genomes of Marek’s disease virus (MDV)，dozens of miRNAs have been identified and some of them are suggested to potentially play important roles in MDV pathogenesis and oncogenesis.Previous studies have shown that the MDV-1-encoded miR-M7-5p is expressed at a high level during different phases of the developing disease.To investigate the potential self-regulation of miR-M7-5p on MDV during virus infection，we have presently scanned the MDV protein-coding genes utilizing bioinformatics method and obtained 10 potential target candidates for miR-M7-5p，including the most important viral oncogene meq.Dual luciferase reporter assay (DLRA) showed that miR-M7-5p can interact with the 3′-UTR of meq gene.Using real-time quantitative RT-PCR，we further confirmed that the transcription level of meq gene in MDV-infected CEF was down regulated by miR-M7-5p.Our data suggests that the oncogene meq is self-regulated by the viral miRNA miR-M7-5p in the infection processes，which may contribute to MDV oncogenesis.

CLC Number:

S852.659.1

ZHAO Pu，TENG Man，LUO Jun，CHI Jia-qi，SU Jing-wei，DANG Lu，DENG Rui-guang，ZHANG Gai-ping. Marek’s Disease Virus Oncogene meq is Targeted by the Viral microRNA miR-M7-5p[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1518-1525.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.09.019

https://www.xmsyxb.com/EN/Y2014/V45/I9/1518

References

［1］CALNEK B W.Pathogenesis of Marek’s disease virus infection［J］.Curr Top Microbiol Immunol，2001,255:25-55.
［2］CHURCHILL A E，PAYNE L N，CHUBB R C.Immunization against Marek’s disease using a live attenuated virus［J］.Nature，1969，221(5182):744-747.
［3］WITTER R L.Increased virulence of Marek’s disease virus field isolates［J］.Avian Dis，1997，41(1):149-163.
［4］GIMENO I M.Marek’s disease vaccines：a solution for today but a worry for tomorrow? ［J］.Vaccine，2008，26 (Suppl 3):C31-41.
［5］OSTERRIEDER N，KAMIL J P，SCHUMACHER D，et al.Marek’s disease virus：from miasma to model［J］.Nat Rev Microbiol，2006，4(4)：283-294.
［6］BARTEL D P.MicroRNAs：genomics，biogenesis，mechanism，and function［J］. Cell，2004，116(2)：281-297.
［7］FILIPOWICZ W，BHATTACHARYYA S N，SONENBERG N.Mechanisms of post-transcriptional regulation by microRNAs：are the answers in sight? ［J］.Nat Rev Genet，2008，9(2)：102-114.
［8］CHO W C.OncomiRs：the discovery and progress of microRNAs in cancers［J/OL］.Mol Cancer，2007，6:60.［2014-06-27］.http://www.molecular-cancer.com/content/6/1/60.
［9］HAGAN J P，CROCE C M.MicroRNAs in carcinogenesis［J］.Cytogenet Genome Res，2007，118(2-4):252-259.
［10］LEWIS B P，BURGE C B，BARTEL D P.Conserved seed pairing，often flanked by adenosines，indicates that thousands of human genes are microRNA targets［J］. Cell，2005，120(1):15-20.
［11］罗俊，滕蔓，樊剑鸣，等.马立克氏病病毒编码的microRNA:从基因组学到功能研究［J］.中国科学：生命科学，2010，40(6)：476-483.
［12］LUO J，SUN A J，TENG M，et al.Expression profiles of microRNAs encoded by the oncogenic Marek’s diseasevirus reveal two distinct expression patterns in vivo during different phases ofdisease［J］.J Gen Virol，2011，92(Pt 3):608-620.
［13］STRASSHEIM S，STIK G，RASSCHAERT D，et al.mdv1-miR-M7-5p，located in the newly identified first intron of the latency-associated transcript of Marek’s disease virus，targets the immediate-early genes ICP4 and ICP27［J］.J Gen Virol，2012，93(Pt 8):1731-1742.
［14］JAROSINSKI K W，TISCHER B K，TRAPP S，et al.Marek’s disease virus：lytic replication，oncogenesis and control［J］.Expert Rev Vaccines，2006，5(6):761-772.
［15］TAM W，DAHLBERG J E.miR-155/BIC as an oncogenic microRNA［J］.Genes Chromosomes Cancer，2006，45(2):211-212.
［16］ZHAO Y，YAO Y，XU H，et al.A functional MicroRNA-155 ortholog encoded by the oncogenic Marek’s disease virus［J］.J Virol，2009，83(1):489-492.
［17］ZHAO Y，XU H，YAO Y，et al.Critical role of the virus-encoded microRNA-155 ortholog in the induction of Marek’s disease lymphomas［J］.PLoS Pathog，2011，7(2):e1001305.
［18］YU Z H，TENG M，SUN A J，et al.Virus-encoded miR-155 ortholog is an important potential regulator but not essential for the development of lymphomas induced by very virulent Marek’s disease virus［J］.Virology, 2014，448:55-64.
［19］XU S，XUE C，LI J，et al.Marek’s disease virus type 1 microRNA miR-M3 suppresses cisplatin-induced apoptosis by targeting Smad2 of the transforming growth factor beta signal pathway［J］.J Virol, 2011，85(1):276-285.

[1]	CHI Lili, WANG Junna, ZHANG Yuming, LIU Jian, CHEN Zhiyuan, LI Shufan, YIN Yanbo, XU Shouzhen. Prokaryotic Expression and Immunogenicity Analysis of Truncated Fusion Protein of FAdV-4 Fiber2 and FAdV-8b Fiber [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5162-5170.
[2]	LI Linyan, TENG Man, LIU Jinling, ZHENG Luping, CHAI Shujun, DING Ke, YU Zuhua, LUO Jun. Development of a Monoclonal Antibody Pool for Marek’s Disease Virus and Identification of a Specific mAb against the Glycoprotein gB [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4712-4723.
[3]	ZHANG Fangyuan, YANG Dawei, QIU Deyang, JIANG Guoqian, LI Guimei, SHAN Hu. Expression of ASFV P30 Protein and Development of ASFV Antibody Detection Method Based on x-MAP Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4300-4310.
[4]	FENG Yongzhi, GONG Ting, WU Dongdong, GAO Qi, ZHENG Xiaoyu, ZHANG Guihong, SUN Yankuo. Analysis of Factors Affecting the Infectivity of African Swine Fever Virus on Cultured Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3406-3414.
[5]	ZHANG Yi, WANG Chenyan, YANG Xiao, SHAO Guoqing, HOU Bo. Phylogenetic Analysis and Pathogenicity of an Avian Infectious Laryngotracheitis Virus Strain WF03 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3435-3444.
[6]	DING Xiaoyan, HE Jiuxiang, ZHOU Xiaoyang, ZHOU Yuxin, LI Jintao. Preliminary Identification of Host Regulatory Genes and Virulence Genes during African Swine Fever Virus Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2964-2971.
[7]	CHEN Hongjian, CAO Yan, FAN Jie, GAN Rongxuan, SONG Wenbo, YU Shengwei, YANG Ting, ZHAO Yanxia, WEI Chunyan, XIE Rui, HUA Lin, PENG Zhong, WU Bin. Prevalence and Phylogenetic Analysis of Pseudorabies Virus within Pig Slaughterhouses in Hubei Province of China during 2020-2022 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2972-2981.
[8]	WANG Jiali, ZHOU Ning, CHEN Xi, YUE Hua, TANG Cheng. Isolation, Identification and Pathogenicity of Canine Adenovirus Type 2 Strain with Natural Deletion in E3 Gene [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2982-2990.
[9]	FENG Weimin, LIU Xiao, HUANG Teng. The Evasion Strategy against CTL Recognition by Herpesviruses of Domestic Animals: Interference with MHC Class Ⅰ Antigen Presentation Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2241-2251.
[10]	GAO Zhenzhen, MENG Zejing, HE Xiaobing, FANG Yongxiang, TIAN Huihui, CHEN Guohua, JING Zhizhong. Identification of Immunogenicity and Neutralizing Activity of Ectromelia Virus EVM135 and EVM085 Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2468-2477.
[11]	WANG Ying, ZHU Jiahong, ZHAO Jiakai, JI Pinpin, CHEN Xu, ZHANG Lu, LIU Baoyuan, SUN Yani, ZHAO Qin. Screening and Identification of Nanobodies against NP419L Protein of African Swine Fever Virus and Its Preliminary Application of Antibody Detection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2509-2520.
[12]	QIU Yingwu, CHANG Hao, PENG Jie, YI Heyou, WANG Qiumei, GUO Yanchen, WU Qianwen, CAO Xuezhen, LIN Limiao, LI Wei, ZHOU Qingfeng, ZHANG Guihong, LI Qunhui, GONG Lang. A Real-time PCR Method for the Rapid Detection of African Swine Fever Virus I177L Gene Deletion Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2521-2527.
[13]	LONG Qinqin, WEI Min, WANG Yuting, WEN Ming, PANG Feng. The Battle between Orf Virus and Host: Immune Response and Viral Immune Evasion Mechanisms [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1845-1853.
[14]	WANG Guochao, ZHAO Yaru, ZHANG Zhonghui, ZHANG Yulong, BAI Ge, GENG Shuxian, FAN Jie, YANG Jifei, GUAN Guiquan, YIN Hong, LUO Jianxun, NIU Qingli. Bioinformatics Analysis of RNA Polymerase Subunit D205R Gene of African Swine Fever Virus and Polyclonal Antibody Preparation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2042-2049.
[15]	YUAN Sheng, LI Anqi, Lü Wenke, YANG Lulu, ZHOU Feng, HUANG Liangzong, BAI Aiquan, WEN Feng, HUANG Shujian, GUO Jinyue. Analysis of Variation in Major Virulence-related Genes of a Strain of Pseudorabies Virus and Its Pathogenicity to Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2195-2199.

Marek’s Disease Virus Oncogene meq is Targeted by the Viral microRNA miR-M7-5p

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments